The Annex Peptides

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blinding
6 min read

How to Blind Vesugen Administration in Rodent Cognitive Studies When Cerebrolysin Is the Active Comparator

Blinding in rodent cognitive studies is a methodological cornerstone. This article defines the steps to achieve effective blinding when Cerebrolysin is the

chronobiology methods
8 min read

How to Control for Circadian Confounders in NAD+ Research

NAD+ levels oscillate with the circadian cycle, confounding research. This protocol uses Epitalon as a positive control to isolate time-of-day effects

FDA compliance
6 min read

How to Stabilize NAD+ in Whole Blood for LC-MS/MS

NAD+ degrades rapidly in whole blood ex vivo. Learn how to optimize sample handling for LC-MS/MS using lessons from MOTS-c stability studies to

blinded crossover trial
4 min read

How to Design a Blinded Crossover Trial for NAD+ and Vesugen Effects on Epigenetic Age Clocks

A protocol framework for a blinded crossover trial comparing NAD+ and Vesugen effects on epigenetic age clocks, covering design, dosing, measurement

LC-MS/MS validation
3 min read

How to Validate NAD+ Quantification in Plasma via LC-MS/MS for Peptide Research

Learn how to validate NAD+ quantification in plasma using LC-MS/MS for peptide research. This guide covers key compounds, consensus methods, active